Understanding follicle growth in vitro: Are we getting closer to obtaining mature oocytes from in vitro-grown follicles in human?
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چکیده
منابع مشابه
P-70: The Effect of Hydrostatic Pressure on Parthenogenetic Activation of Mouse Oocytes Derived from In vitro Grown Ovarian Follicles
Background: Parthenogenesis is the production of an embryo from a female gamete in the absence of any contribution from a male gamete, with or without the eventual development into an adult. In vivo, mammalian parthenogenesis is a rare event. Parthenogenesis can be efficiently induced in vitro with a variety of mechanical, chemical, and electrical stimuli in several species. Hydrostatic pressur...
متن کاملP-120: Meiotic Spindle Visualization and Zona Pellucida Birefringence in Relation to Morphology of In Vivo and In Vitro Matured Human Oocytes of In Vivo and In Vitro Matured Human Oocytesof In Vivo and In Vitro Matured Human Oocytes
Background: The meiotic spindle plays an important role in the oocyte during chromosome alignment and separation at meiosis. The zona pellucid (ZP) is a dynamic matrix composed of filaments with the properties that might reflect the history of oocyte cytoplasmic maturation. Since, spindle and ZP in living oocytes are highly birefringent, their structures can be viewed noninvasively by using a P...
متن کاملIn vitro grown human ovarian follicles from cancer patients support oocyte growth.
BACKGROUND Young female adult and adolescent cancer patients facing life-preserving but fertility-threatening chemo- or radiation-therapy are increasingly seeking options to protect their reproductive potential. Ovarian tissue cryopreservation with transplantation is a promising technique to safeguard fertility in cancer patients. However, this method may risk re-introduction of the original ca...
متن کاملRole of Follicle Stimulating Hormone in the Survival, Activation and Further Growth of in vitro Cultured Sheep Primordial Follicles
The aim of the present study was to investigate the effect of follicle stimulating hormone (FSH) on survival, activation and growth of ovine primordial follicles using histological studies. Pieces of ovine ovarian cortex were obtained and cultured for 6 days in Minimum Essential Medium supplemented with or without FSH (50 ng/mL). These fragments were then process to be used for histology compar...
متن کاملGrowth of Ovarian Primary Follicles Retrieved from Neonates of Different Ages and Derivation of Mature Oocytes Following In vitro-Culture
This study was conducted to improve the yield of mature oocytes from in vitro-culture of ovarian primary follicles by optimizing follicle retrieval from neonatal mice of different ages. Primary follicles of 75 to 99 μm in diameter were collected daily from 7- to 14-day-old neonatal mice, and subsequently cultured in α-MEM medium. Number of primary follicles isolated, growth of the follicle duri...
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ژورنال
عنوان ژورنال: Molecular Reproduction and Development
سال: 2017
ISSN: 1040-452X
DOI: 10.1002/mrd.22822